Aerosol activity of forming cloud droplets affects cloud micro-physics and indicates aerosol hygroscopicity. Application of the cloud condensation nuclei (CCN) counter (CCNC) is an important method for studying CCN-active particles. Factors impacting measurements using CCNC are analyzed by laboratory study. In CCNC, variations of supre-saturations (SS) are found to be proportional to variations of the pressure and the flow-rate. Temperature conditions, under which CCNC is working, exhibit nonlinear influnces on SSs in CCNC. Varying aerosol losses with particle size due to the pressure adjustment is found when differential pressure is higher than 300 hPa. Underestimation of CCN number concentration is found when particle number concentration is higher than 10000/cm3 and SS in CCNC is lower than 0.2%. This is because only part of CCN-active particles can activate due to the vapor depletion in CCNC. The results and suggestions provide instructions for CCN measurment and would help improving quality control and analysis of CCN data.